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2009-09-30Zeitschriftenartikel DOI: 10.1128/CVI.00235-09
Development of Antibodies against Anthrose Tetrasaccharide for Specific Detection of Bacillus anthracis Spores
dc.contributor.authorKühn, Andrea
dc.contributor.authorKovac, Pavol
dc.contributor.authorSaksena, Rina
dc.contributor.authorBannert, Norbert
dc.contributor.authorKlee, Silke
dc.contributor.authorRanisch, Heidrun
dc.contributor.authorGrunow, Roland
dc.date.accessioned2018-05-07T14:00:51Z
dc.date.available2018-05-07T14:00:51Z
dc.date.created2010-06-22
dc.date.issued2009-09-30none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rextM8ugy3A3E/PDF/26Vu3RXbOVE6c.pdf
dc.identifier.urihttp://edoc.rki.de/176904/684
dc.description.abstractMethods for the immunological detection of Bacillus anthracis in various environmental samples and the discrimination of B. anthracis from other members of the B. cereus group are not yet well established. To generate specific discriminating antibodies, we immunized rabbits, mice, and chickens with inactivated B. anthracis spores and, additionally, immunized rabbits and mice with the tetrasaccharide beta-Ant-(1-->3)-alpha-L-Rhap-(1-->3)-alpha-L-Rhap-(1-->2)-L-Rhap. It is a constituent of the exosporium glycoprotein BclA and contains the newly discovered sugar anthrose 2-O-methyl-4-(3-hydroxy-3-methylbutamido)-4,6-dideoxy-beta-D-glucose. The BclA protein is a major component of the exosporium of B. anthracis spores and is decorated by the tetrasaccharide indicated above. The anthrose-containing tetrasaccharide chain seems to be highly specific for B. anthracis, which makes it a key biomarker for the detection of these spores. The different immunizations led to anthrose-reactive polyclonal and monoclonal antibodies which were analyzed by various methods to characterize their ability to discriminate between B. anthracis and other Bacillus spp. Multiple applications, such as enzyme-linked immunosorbent assay, indirect immunofluorescence assay, and electron microscopy, revealed the specificities of the polyclonal and monoclonal antibodies generated for B. anthracis spore detection. All polyclonal antibodies were able to correctly identify the B. anthracis strains tested and showed only minimal cross-reactivities with other Bacillus strains. Moreover, the antibodies generated proved functional in a new capture assay for B. anthracis spores and could therefore be useful for the detection of spores in complex samples.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectFemaleeng
dc.subjectMiceeng
dc.subjectMicroscopyeng
dc.subjectMembrane Glycoproteins/metabolismeng
dc.subjectAntibodieseng
dc.subjectBacillus anthracis/isolation & purificationeng
dc.subject* Amino Sugars/immunology*eng
dc.subject* Animalseng
dc.subject* Anthrax/immunology*eng
dc.subjectAnthrax/microbiologyeng
dc.subjectBacterial/immunologyeng
dc.subjectBacterial/metabolismeng
dc.subjectBacillus anthracis/immunologyeng
dc.subjectDeoxyglucose/analogs & derivativeseng
dc.subjectDeoxyglucose/immunologyeng
dc.subjectMembrane Glycoproteins/immunologyeng
dc.subjectInbred BALB Ceng
dc.subjectImmunoelectroneng
dc.subjectRabbitseng
dc.subjectSporeseng
dc.subjectBacterial/isolation & purificationeng
dc.subjectBacterial/ultrastructureeng
dc.subject.ddc610 Medizin
dc.titleDevelopment of Antibodies against Anthrose Tetrasaccharide for Specific Detection of Bacillus anthracis Spores
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-1009493
dc.identifier.doi10.1128/CVI.00235-09
dc.identifier.doihttp://dx.doi.org/10.25646/609
local.edoc.container-titleClinical and Vaccine Immunology
local.edoc.container-textKuehn, A., Kovác, P., Saksena, R., Bannert, N., Klee, S.R., Ranisch, H., Grunow, R. Development of antibodies against anthrose tetrasaccharide for specific detection of Bacillus anthracis spores (2009) Clinical and Vaccine Immunology, 16 (12), pp. 1728-1737.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://cdli.asm.org/cgi/content/abstract/16/12/1728
local.edoc.container-publisher-nameAmerican Society for Microbiology
local.edoc.container-volume16
local.edoc.container-issue12
local.edoc.container-year2009

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