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2010-09-14Zeitschriftenartikel DOI: 10.1186/1743-422X-7-226
Phylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia
dc.contributor.authorMendez, Jairo A.
dc.contributor.authorUsme-Ciro, Jose A.
dc.contributor.authorDomingo, Cristina
dc.contributor.authorRey, Gloria J.
dc.contributor.authorSanchez, Juan A.
dc.contributor.authorTenorio, Antonio
dc.contributor.authorGallego-Gomez, Juan C.
dc.date.accessioned2018-05-07T14:14:32Z
dc.date.available2018-05-07T14:14:32Z
dc.date.created2010-11-25
dc.date.issued2010-09-14none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reOrlQ7Rr93E/PDF/22jIS8l66muoM.pdf
dc.identifier.urihttp://edoc.rki.de/176904/757
dc.description.abstractBackground: Dengue Fever is one of the most important viral re-emergent diseases affecting about 50 million people around the world especially in tropical and sub-tropical countries. In Colombia, the virus was first detected in the earliest 70′s when the disease became a major public health concern. Since then, all four serotypes of the virus have been reported. Although most of the huge outbreaks reported in this country have involved dengue virus serotype 1 (DENV-1), there are not studies about its origin, genetic diversity and distribution. Results: We used 224 bp corresponding to the carboxyl terminus of envelope (E) gene from 74 Colombian isolates in order to reconstruct phylogenetic relationships and to estimate time divergences. Analyzed DENV-1 Colombian isolates belonged to the formerly defined genotype V. Only one virus isolate was clasified in the genotype I, likely representing a sole introduction that did not spread. The oldest strains were closely related to those detected for the first time in America in 1977 from the Caribbean and were detected for two years until their disappearance about six years later. Around 1987, a split up generated 2 lineages that have been evolving separately, although not major aminoacid changes in the analyzed region were found. Conclusion: DENV-1 has been circulating since 1978 in Colombia. Yet, the phylogenetic relationships between strains isolated along the covered period of time suggests that viral strains detected in some years, although belonging to the same genotype V, have different recent origins corresponding to multiple re-introduction events of viral strains that were circulating in neighbor countries. Viral strains used in the present study did not form a monophyletic group, which is evidence of a polyphyletic origin. We report the rapid spread patterns and high evolution rate of the different DENV-1 lineages.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectMoleculareng
dc.subjectDNAeng
dc.subjectGeneticeng
dc.subjectHumanseng
dc.subjectPhylogenyeng
dc.subjectGenotypeeng
dc.subjectRNAeng
dc.subjectCluster Analysiseng
dc.subjectPolymorphismeng
dc.subjectViral/geneticseng
dc.subjectSequence Analysiseng
dc.subjectMolecular Epidemiologyeng
dc.subjectViral Envelope Proteins/geneticseng
dc.subjectEvolutioneng
dc.subjectColombia/epidemiologyeng
dc.subjectDengue/epidemiologyeng
dc.subjectDengue/virologyeng
dc.subjectDengue Virus/classificationeng
dc.subjectDengue Virus/geneticseng
dc.subjectDengue Virus/isolation & purificationeng
dc.subject.ddc610 Medizin
dc.titlePhylogenetic history demonstrates two different lineages of dengue type 1 virus in Colombia
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10011596
dc.identifier.doi10.1186/1743-422X-7-226
dc.identifier.doihttp://dx.doi.org/10.25646/682
local.edoc.container-titleVirology Journal
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.virologyj.com/content/7/1/226
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume7
local.edoc.container-issue226
local.edoc.container-year2010

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