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2009-12-18Zeitschriftenartikel DOI: 10.1099/ijs.0.019026-0
Orbus hercynius gen. nov., sp. nov., isolated from faeces of wild boar, is most related to Enterobacteriales and Pasteurellales
dc.contributor.authorVolkmann, Maria
dc.contributor.authorSkiebe, Evelyn
dc.contributor.authorKerrinnes, Tobias
dc.contributor.authorFaber, Franziska
dc.contributor.authorLepka, Daniela
dc.contributor.authorPfeifer, Yvonne
dc.contributor.authorHolland, Gudrun
dc.contributor.authorBannert, Norbert
dc.contributor.authorWilharm, Gottfried
dc.date.accessioned2018-05-07T14:18:33Z
dc.date.available2018-05-07T14:18:33Z
dc.date.created2010-12-20
dc.date.issued2009-12-18none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re7Kg83PMgve/PDF/20DJtR77r2M46.pdf
dc.identifier.urihttp://edoc.rki.de/176904/778
dc.description.abstractA novel gammaproteobacterium, strain CN3T, was isolated from faeces of wild boar. It is facultative anaerobic and appears coccoid or rod shaped. The determined partial 16S rRNA gene sequence of strain CN3T suggests a distant relationship to Enterobacteriales and Pasteurellales. The sequence shows highest similarity of 90.3% with Obesumbacterium proteus DSM 2777T, a member of the Enterobacteriaceae. The closest relatives outside the Enterobacteriales according to 16S rRNA gene sequence analysis are members of the Pasteurellales with 88.7% similarity (Mannheimia haemolytica NCTC 9380T and Actinobacillus lignieresii NCTC 4189T). In contrast to most members of the Enterobacteriales, strain CN3T is oxidase-positive. The pattern of fatty acids, in particular the high relative abundance of C18:1{omega}7c (38.5%), is clearly distinct from the conserved pattern of Pasteurellales. EcoRI ribotyping of strain CN3T yielded no significant similarity to database entries. Major ubiquinone of strain CN3T is Q-8. The DNA G+C content is 36.4 mol%. CN3T hosts a phage and secretes considerable amounts of three proteins into the culture supernatant. A spontaneous mutant of strain CN3T was isolated forming long filaments. Microscopic studies revealed the presence of a capsule which the mutant strain is unable to partition after cell division. CN3T (=DSM 22228T=CCUG 57622T) is considered as the type strain of a novel species within a new genus, for which the name Orbus hercynius gen. nov., sp. nov. is proposed. Its classification to family and order requires further investigation.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectAnimalseng
dc.subjectMolecular Sequence Dataeng
dc.subjectPhylogenyeng
dc.subjectFeces/microbiologyeng
dc.subjectDNA Bacterial/geneticseng
dc.subjectBase Compositioneng
dc.subjectFatty Acids/metabolismeng
dc.subjectGammaproteobacteria/classificationeng
dc.subjectGammaproteobacteria/geneticseng
dc.subjectGammaproteobacteria/isolation & purificationeng
dc.subjectGammaproteobacteria/metabolismeng
dc.subjectRNA Ribosomal 16S/geneticseng
dc.subjectSus scrofa/microbiologyeng
dc.subject.ddc610 Medizin
dc.titleOrbus hercynius gen. nov., sp. nov., isolated from faeces of wild boar, is most related to Enterobacteriales and Pasteurellales
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10011947
dc.identifier.doi10.1099/ijs.0.019026-0
dc.identifier.doihttp://dx.doi.org/10.25646/703
local.edoc.container-titleInternational Journal of Systematic and Evolutionary Microbiology
local.edoc.container-textThis is an author manuscript that has been accepted for publication in Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of ‘Fair Use of Copyrighted Materials’ (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://mic.sgmjournals.org, and is freely available without a subscription 12 months after publication.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://ijs.sgmjournals.org/cgi/content/abstract/ijs.0.019026-0v1
local.edoc.container-publisher-nameSociety for General Microbiology
local.edoc.container-volume60
local.edoc.container-issue11
local.edoc.container-year2009

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