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Auflistung Artikel in Fachzeitschriften nach Autor "Gerlach, Roman"
Anzeige der Publikationen 1-9 von 9
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2015-04-09ZeitschriftenartikelA Gateway-Based System for Fast Evaluation of Protein-Protein Interactions in Bacteria Wille, Thorsten; Barlag, Britta; Jakovljevic, Vladimir; Hensel, Michael; Sourjik, Victor; Gerlach, RomanProtein-protein interactions are important layers of regulation in all kingdoms of life. Identification and characterization of these interactions is one challenging task of the post-genomic era and crucial for understanding ...
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2010-01-05ZeitschriftenartikelCorynebacterium diphtheriae invasion-associated protein (DIP1281) is involved in cell surface organization, adhesion and internalization in epithelial cells Ott, Lisa; Höller, Martina; Gerlach, Roman; Hensel, Michael; Rheinlaender, Johannes; Schäffer, Tilman E; Burkovski, AndreasBackground: Corynebacterium diphtheriae, the causative agent of diphtheria, is well-investigated in respect to toxin production, while little is known about C. diphtheriae factors crucial for colonization of the host. In ...
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2015-06-16ZeitschriftenartikelCross talk between the response regulators PhoB and TctD allows for the integration of diverse environmental signals in Pseudomonas aeruginosa Bielecki, Piotr; Jensen, Vanessa; Schulze, Wiebke; Gödeke, Julia; Strehmel, Janine; Eckweiler, Denitsa; Nicolai, Tanja; Bielecka, Agata; Wille, Thorsten; Gerlach, Roman; Häussler, SusanneTwo-component systems (TCS) serve as stimulus-response coupling mechanisms to allow organisms to adapt to a variety of environmental conditions. The opportunistic pathogen Pseudomonas aeruginosa encodes for more than 100 ...
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2011-10-21ZeitschriftenartikelGaussia princeps luciferase (Gluc) as reporter for transcriptional activity, protein secretion and protein-protein interactions in Salmonella enterica serovar Typhimurium Wille, Thorsten; Blank, Kathrin; Schmidt, Christiane; Vogt, Vivien; Gerlach, RomanGaussia princeps luciferase (Gluc) is widely used as a reporter in eukaryotes, but data about its applicability in bacteria is very limited. Here we show that a codon-optimized Gluc gene can be efficiently expressed in ...
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2014-01-02ZeitschriftenartikelInflammation Fuels Colicin Ib-Dependent Competition of Salmonella Serovar Typhimurium and E. coli in Enterobacterial Blooms Nedialkova, Lubov Petkova; Denzler, Rémy; Koeppel, Martin B.; Diehl, Manuel; Ring, Diana; Wille, Thorsten; Gerlach, Roman; Stecher, BärbelThe host's immune system plays a key role in modulating growth of pathogens and the intestinal microbiota in the gut. In particular, inflammatory bowel disorders and pathogen infections induce shifts of the resident commensal ...
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2013-08-08ZeitschriftenartikelLPS Structure and PhoQ Activity Are Important for Salmonella Typhimurium Virulence in the Gallleria mellonella Infection Model Bender, Jennifer K.; Wille, Thorsten; Blank, Kathrin; Lange, Anna; Gerlach, RomanThe larvae of the wax moth, Galleria mellonella, have been used experimentally to host a range of bacterial and fungal pathogens. In this study we evaluated the suitability of G. mellonella as an alternative animal model ...
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2014-03-18ZeitschriftenartikelPre- and Post-Processing Workflow for Affinity Purification Mass Spectrometry Data Fischer, Martina; Zilkenat, Susann; Gerlach, Roman; Wagner, Samuel; Renard, Bernhard Y.The reliable detection of protein–protein interactions by affinity purification mass spectrometry (AP-MS) is crucial for the understanding of biological processes. Quantitative information can be used to separate truly ...
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2011-01-14ZeitschriftenartikelRapid and Highly Efficient Method for Scarless Mutagenesis within the Salmonella enterica Chromosome Blank, Kathrin; Hensel, Michael; Gerlach, RomanDirect manipulation of bacterial chromosomes by recombination-based techniques has become increasingly important for both cognitive and applied research. Here we demonstrate, for the first time, the combination of the Red ...
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2013-02-05ZeitschriftenartikelSite-Directed Mutagenesis Using Oligonucleotide-Based Recombineering Gerlach, Roman; Blank, Kathrin; Wille, Thorsten