Browsing Artikel in Fachzeitschriften by Subject "Sensitivity and Specificity"
Now showing items 1-20 of 44
-
2010-10-05Zeitschriftenartikel2nd International External Quality Control Assessment for the Molecular Diagnosis of Dengue Infections Background: Currently dengue viruses (DENV) pose an increasing threat to over 2.5 billion people in over 100 tropical and sub-tropical countries worldwide. International air travel is facilitating rapid global movement ...
-
2010-07-01ZeitschriftenartikelAbsence of evidence of Xenotropic Murine Leukemia Virus-related virus infection in persons with Chronic Fatigue Syndrome and healthy controls in the United States Background: XMRV, a xenotropic murine leukemia virus (MuLV)-related virus, was recently identified by PCR testing in 67% of persons with chronic fatigue syndrome (CFS) and in 3.7% of healthy persons from the United States. ...
-
2008-12-01ZeitschriftenartikelAntibodies against Rickettsia spp. in Hunters, Germany
-
2011-10-20ZeitschriftenartikelComparison of the novel ResPlex III assay and existing techniques for the detection and subtyping of influenza virus during the influenza season 2006–2007 Influenza virus is a major cause of disease worldwide. The accurate detection and further subtyping of influenza A viruses are important for epidemiologic surveillance, and subsequent comprehensive characterization of ...
-
2003-05-02ZeitschriftenartikelComparison of two DNA targets for the diagnosis of Toxoplasmosis by real-time PCR using fluorescence resonance energy transfer hybridization probes Background: Toxoplasmosis is an infectious disease caused by the parasitic protozoan Toxoplasma gondii. It is endemic worldwide and, depending on the geographic location, 15 to 85% of the human population are asymptomatically ...
-
2012-09-27ZeitschriftenartikelConfidence-based Somatic Mutation Evaluation and Prioritization Next generation sequencing (NGS) has enabled high throughput discovery of somatic mutations. Detection depends on experimental design, lab platforms, parameters and analysis algorithms. However, NGS-based somatic mutation ...
-
2009-03-01ZeitschriftenartikelCoordinated Implementation of Chikungunya Virus Reverse Transcription–PCR. A preformulated chikungunya virus real-time reverse transcription-PCR, quality-confirmed oligonucleotides, and noninfectious virus controls were distributed by the European Network for the Diagnosis of Imported Viral ...
-
2008-12-03ZeitschriftenartikelDetection of Balamuthia mandrillaris DNA by real-time PCR targeting the RNase P gene Background: The free-living amoeba Balamuthia mandrillaris may cause fatal encephalitis both in immunocompromised and in – apparently – immunocompetent humans and other mammalian species. Rapid, specific, sensitive, and ...
-
2006-07-01ZeitschriftenartikelDetection of Infectious Poxvirus Particles. To enable rapid and reliable detection of poxviruses in clinical and environmental specimens, a diagnostic approach was developed to detect
-
2006-04-10ZeitschriftenartikelDetection of Norovirus genogroup I and II by multiplex real-time RT- PCR using a 3'-minor groove binder-DNA probe Background: Due to an increasing number of norovirus infections in the last years rapid, specific, and sensitive diagnostic tools are needed. Reverse transcriptase-polymerase chain reactions (RT-PCR) have become the methods ...
-
2014-09-29ZeitschriftenartikelDetection of OXA-48-type carbapenemase-producing Enterobacteriaceae in diagnostic laboratories can be enhanced by addition of bicarbonates to cultivation media or reaction buffers Carbapenemase-mediated resistance to carbapenems in Enterobacteriaceae has become the main challenge in the treatment and prevention of infections recently. The partially unnoticed spread of OXA-48-type carbapenemase ...
-
2015-11-05ZeitschriftenartikelDevelopment and deployment of a rapid recombinase polymerase amplification Ebola virus detection assay in Guinea in 2015 In the absence of a vaccine or specific treatments for Ebola virus disease (EVD), early identification of cases is crucial for the control of EVD epidemics. We evaluated a new extraction kit (SpeedXtract (SE), Qiagen) on ...
-
2010-01-27ZeitschriftenartikelDifferentiation of influenza b virus lineages yamagata and victoria by real-time PCR Since the 1970s, influenza B viruses have diverged into two antigenically distinct virus lineages called the Yamagata and Victoria lineages. We present the first real-time PCR assay for virus lineage differentiation to ...
-
2012-03-13ZeitschriftenartikelEnhanced fungal DNA-extraction from formalin-fixed, paraffin-embedded tissue specimens by application of thermal energy Determining the etiology of invasive fungal infections (IFI) is critical for patient management as fungi vary in their susceptibility to antifungals. However, the etiology remains obscure in many cases due to negative ...
-
2011-12-27ZeitschriftenartikelEvaluating frequency, diagnostic quality, and cost of lyme borreliosis testing in Germany: a retrospective model analysis. Background: Data on the economic impact of Lyme borreliosis (LB) on European health care systems is scarce. This project focused on the epidemiology and costs for laboratory testing in LB patients in Germany. Materials ...
-
2007-07-03ZeitschriftenartikelFirst international diagnostic accuracy study for the serological detection of West Nile virus infection Background: The diagnosis of an acute or convalescent West Nile (WN) virus infection can be confirmed by various serological assays such as enzyme immunoassay (EIA), immunofluorescence assay (IFA), or neutralisation test ...
-
2012-05-03ZeitschriftenartikelFirst International External Quality Assessment Study on Molecular and Serological Methods for Yellow Fever Diagnosis Objective: We describe an external quality assurance (EQA) study designed to assess the efficiency and accurateness of molecular and serological methods used by expert laboratories performing YF diagnosis. Study Design: ...
-
2009-11-26ZeitschriftenartikelHigh genotypic diversity and a novel variant of human cytomegalovirus revealed by combined UL33/UL55 genotyping with broad-range PCR The known strains of human cytomegalovirus (HCMV) represent genotypic variants of a single species, and HCMV genotypic variability has been studied in order to reveal correlations between different disease patterns and the ...
-
2011-02-10ZeitschriftenartikelHighly sensitive detection of the group A Rotavirus using Apolipoprotein H-coated ELISA plates compared to quantitative real-time PCR Background: The principle of a capture ELISA is binding of specific capture antibodies (polyclonal or monoclonal) to the surface of a suitable 96 well plate. These immobilized antibodies are capable of specifically binding ...
-
2006-01-19ZeitschriftenartikelHighly sensitive real-time PCR for specific detection and quantification of Coxiella burnetii Background: Coxiella burnetii, the bacterium causing Q fever, is an obligate intracellular biosafety level 3 agent. Detection and quantification of these bacteria with conventional methods is time consuming and dangerous. ...